Isoelectric point calculations are critical in your biochemistry course and MCAT prep. The isoelectric point or pI gives you the pH at which the molecule has a net zero charge. This comes from a balance of protonation and deprotonation so that all positive and negative charges cancel out.
This video shows you how to calculate amino acid isoelectric point along with a shortcut to help you figure out WHICH pKa to use when given a side chain pKa value.
(Watch on YouTube: Isoelectric Point of Amino Acids. Click cc on the bottom right for video transcript)
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This is video 6 in the Amino Acids Series. Click for complete series + Practice Quiz and Cheat Sheet
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Why is Tyrosine an acidic amino acid?
You said the rule for finding the PI for aa’s with proton-able side chains is: for an acidic aa (Asp, Glu, Cys, Tyr) use the pKa of the carboxy and the R-group, and for a basic aa (His, Lys, Arg) use the pKa of the amine and the R-group.
However in the previous example the pKa of the side chain of Tyr was above that of the amine and carboxy, and we did not use the pKa of that value to find the PI. Does this aa not follow the rule / Tyr is not an acidic aa?
No one… I repeat no one can explain this like you! I have your voice in my head as I study these lol!
This is the most amazing video I’ve ever learned from. Some prep companies failed to explain this fully like you did, and I found myself making the mistakes you mentioned! This is great, wish I found it a bit earlier.
Rim, I’m glad this has been helpful to you! I was a student once too, so I know exactly what you mean. I try to provide the resources I wish I could have had. How long until you have your MCAT exam?